The Comparison of Oxidant and Antioxidant Parameters in Inflamed Paw Tissue Formed by Carrageenan Injection in Intact and Adrenalectomized Rats

Aim: In this study, oxidative stress, antioxidant response and inflammation parameters were investigated in intact and carrageenan-inflamed paw tissues in intact and adrenalectomized rats. The effects of prednisolone and adrenaline on these parameters were investigated. Materials and Methods: 37 Albino Wistar male rats were divided into six groups as follows: I: only adrenalectomized, II: adrenalectomized + carrageenan-given, III: adrenalectomized + prednisolone-and carrageenan-given, IV: adrenalectomized + adrenalineand carrageenan-given, V: only carrageenan-given and VI: intact rats. Carrageenan was injected as the inflammatory agent. At the end of experiments, the paw tissues were obtained and homogenised. Thiobarbituric acid reacting substances and nitric oxide levels, myeloperoxidase, superoxide dismutase, glutathione peroxidase and glutathione S-transferase activities were measured in the supernatants. Results: The highest mean thiobarbituric acid reacting substances and nitric oxide, the lowest superoxide dismutase and glutathione peroxidase values were determined in group II. In group III and IV were significantly suppressed thiobarbituric acid reacting substances, nitric oxide and myeloperoxidase values and increased superoxide dismutase, glutathione peroxidase and glutathione S-transferase activities. On the other hand, there was not any statistically significant difference between group III and IV’s parameters. Conclusion: These results showed that adrenalectomy resulted increase in oxidant parameters and decrease in antioxidant parameters, particularly in inflammation. Prednisolone and adrenaline significantly suppressed the increase in oxidants and decrease in antioxidants in adrenalectomized + carrageenan-given rats.